Abstract
ABSTRACTThis study investigated the effects of copper oxide nanoparticles (CuO NPs) on the growth and development of transgenic cotton harboring the Ipt gene, which encodes isopentenyl transferase (Ipt). Three concentrations of CuO NPs were evaluated: 10, 200, and 1000 mg·L-1, each with three replicates. The height and the root length were 26.91% and 42.80% decreased after 10-day exposure with 1000 mg·L-1 CuO NPs, respectively.In addition, less abundant on root hairs and lower in shoot biomass of Ipt-cotton when compared with the control group. The growth of Ipt-cotton was not affected by 10 mg·L-1 CuO NPs, but a high concentration of CuO NPs promoted the absorption of Fe and Na into roots, and inhibited the production of phytohormones in Ipt-cotton. The CuO NPs increased the concentration of iPA in shoots, which can delay senescence. The extent of the increase in iPA in response to CuO NPs should be relative to the amount of Ipt immobilized onto the NPs in the plant tissue. To our knowledge, this is the first study to evaluate the phytotoxicity of CuO NPs to Ipt-transgenic cotton. These results establish a baseline for further research on the effects of nanoparticles on transgenic crops harboring the Ipt gene.
Highlights
Copper oxide nanoparticles (CuO NPs) have been one of the most widely used metal nanoparticles (NPs) since 2011, and have attracted much interest because of their catalytic, electric, optical, photonic, textile, and nanofluid properties, and their antibacterial activity (Perelshtein et al 2009; Devi & Singh 2014; Tamuly et al 2014)
The extent of the increase in isopentenyl adenine (iPA) in response to CuO NPs should be relative to the amount of isopentenyl transferase (Ipt) immobilized onto the NPs in the plant tissue. This is the first study to evaluate the phytotoxicity of CuO NPs to Ipt-transgenic cotton
These results showed that plant height, root length, and the number of root hairs were affected by CuO NPs at high concentrations, but not by CuO NPs at low concentrations
Summary
Copper oxide nanoparticles (CuO NPs) have been one of the most widely used metal nanoparticles (NPs) since 2011, and have attracted much interest because of their catalytic, electric, optical, photonic, textile, and nanofluid properties, and their antibacterial activity (Perelshtein et al 2009; Devi & Singh 2014; Tamuly et al 2014). The CuO NPs and other Cu complexes have been used for various purposes: as water purifiers, algaecides, fungicides, antibacterial compounds, and antifouling agents (Raul et al 2014). NPs have been shown to have negative effects on plants. Alumina NPs inhibited root elongation in corn, cucumber, soybean, cabbage, and carrot (ma et al 2010). The growth of Saccharomyces cerevisiae BY4741 was inhibited by CuO NPs (Gregersen et al 2013). These studies have increased our knowledge about the phytotoxicity of NPs; less is known about the phytotoxic effects of lower concentrations of NPs and the environment impacts of nanoparticles remain uncertain (Syu et al 2014)
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