Abstract
This study focused on the effects of the insect growth regulator diflubenzuron (DFB) on nauplii of the estuarine copepod Eurytemora affinis. The 48-h LC50 for DFB and E. affinis nauplii was 2·2 μg/liter DFB. Short-term effects on naupliar survival and development to the copepodite stage were observed following 5 or 6 days exposure to 0·78 μg/liter DFB. These results suggest that DFB has substantial effects on the survival and development of E. affinis at water concentrations of less than 1 μg/liter. The test system included algae and clay added to mimic the maximum amount of kaolin present in the pesticide formulation. These effects may adversely impact natural zooplankton populations which are an important component of the estuarine food chain in Chesapeake Bay and elsewhere. Due to reports of DFB concentrations occasionally exceeding 1 μg/liter in some Chesapeake Bay tributary surface waters, DFB represents a potential risk to E. affinis.
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