Toxic effects of pentachlorophenol and 2,2',4,4'-tetrabromodiphenyl ether on two generations of Folsomia candida

Abstract

The standard Folsomia candida test (ISO 11267), in which only the survival and reproduction of the parental generation (F0) were determined, is insufficient to assess the toxicity of chemicals, like endocrine disrupting chemicals (EDCs), since the effects of EDCs could last for several generations and sometimes can be transgenerational. It's necessary to assess the effects on subsequent generations to address the long-term consequences of these chemicals exposure. In this study, the effects of pentachlorophenol (PCP) and 2,2',4,4'-tetrabromodiphenyl (BDE47) were assessed on F0 and the first filial generation (F1) of F. candida after 28-day or 10-day exposure of F0. In the 28-day exposure method, F0 was exposed to PCP or BD47 for 28 days and F1 was exposed for about 21 days. In the 10-day exposure method, F0 was exposed for 10 days and F1 was not exposed. The F. candida reproduction of F0 and F1 can be assessed in both methods, while transgenerational effects can further be evaluated in the 10-day exposure method. The numbers of F1 and F2 (second filial generation) juveniles in the 28-day exposure method and F1 juveniles in the 10-day exposure method decreased significantly for the PCP treatment. For BDE47, only the number of F1 juveniles in the 28-day exposure method significantly decreased. The EC50 values of F0 reproduction (the number of F1 juveniles) in the 28-day exposure method were 89 and 306 mg/kg dry soil for PCP and BDE47, respectively. Results suggested that PCP could affect F. candida egg hatching or juvenile survival and adult reproductive capacity, while BDE47 was more likely to affect egg hatchability or juvenile survival rather than adult reproductive capacity. It also indicated that F. candida exposed to PCP or BDE47 could recover in clean soil. Transgenerational effects were not observed for neither PCP nor BDE47 in this study.