Toxic effects of ornamental stone processing waste effluents on Geophagus brasiliensis (Teleostei: Cichlidae)

Abstract

The ornamental stone industry generates considerable amounts of waste (OSPW), which may eventually reach natural environments and impact the local ecosystem. The aim of this study was to compare the toxic effects of two OSPW effluents in Geophagus brasiliensis: i) leachate effluent from a lagoon in an OSPW landfill (LE) and ii) decanted effluent from an ornamental stone processing industry (DE). G. brasiliensis were submitted to acute contamination with both OSPW effluents. After contamination, the gills were extracted for evaluation of histopathological alterations and ion concentration, while the liver underwent catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and glutathione s-transferase (GST) enzyme activity analysis. An induced biomarker response (IBRv2) index was determined to correlate the multi-biomarker response in G. brasiliensis. Fish gills exposed to DE showed increased concentration of Ca2+, Mg2+, Na+, and K+ when compared to those treated with LE. Histopathological lesions were observed in gills of animals exposed to both effluents. Micronucleus and comet assay were significantly greater in fish exposed to DE, when compared to those contaminated with LE. The evaluation of the enzymatic activity of CAT, GPx and SOD indicate greater oxidative stress in DE and LE-exposed fish, while GST activity was not altered. DE showed an IBRv2 value almost two-times higher in relation to LE, indicating that this waste may present higher toxic potential. The results demonstrate that both contaminants led to substantial toxic effects in G. brasiliensis, although the decanted waste induced the most remarkable responses in G. brasiliensis.