Toxic Effects of Magnesium Nitrate on Cardiac Muscle Tissue of Gallus Domesticus Embryos and Chicks

Abstract

Eggs from avian hybrid ROSS-308 have undergone incubation process, after previously being controlled, sanitized, measured and weighed. We made three groups of eggs, one control (LM) and two experimental (LE1 and LE2).The 3 groups were homogeneous, both as a weight (59.47 to 62.371 g) and volume (55.417 to 58.177 cm3) and surface (59.315 to 72.305 cm2). The eggs of the two experimental groups were injected 0.1 mL of a solution of magnesium nitrate, solution which had a concentration of 0.16%, for LE1 and of 0.61% for LE2. The timing of injection of the solution was at 2, 4, 6 and 8 days after the beginning of the incubation process, for both experimental groups. At the end of incubation period we studied both embryos and the resulting offspring. They were weighed, measured and evaluated in terms of necropsy, and some of them were slaughtered and dissected, extracting from them a series of body organs such as the heart, liver, stomach and intestinal mass. From the heart some histological samples were taken which were processed by paraffin sectioning techniques to yield 20 three-color (HEA) stained histological slides. These samples were studied by optic microscopy and we highlighted the modifications that appeared to the myocardial tissue. Heart weighted between 0.3308 to 0.3843 g, representing 0.807 to 1.062% of the body weight. Myocardial tissue has undergone significant pathological changes such as metaplasia of the vascular wall, perivascular edema, disintegration and degeneration of muscle cells and myocardial steatosis.