Toxic effects of Cyclophosphamide on Hepatic and Kidney tissues in Albino Mice Model

Abstract

cyclophosphamide (CPA) is anti-tumor drug and is used for chemotherapy. CPA is a poisonous medication that hits both tumor and healthy cells in the body. The active ingredient is inactive in vitro and is replaced by metabolites, the most important of which is phosphoramide mustard. The goal of this study was to see how cyclophosphamide affects the histology of the liver and kidney tissues in mice animal model, as well as how it affects their biochemical functions. Twenty adult male Albino mice were randomly assigned to control and CPA treated group. CPA was given to the mice at dose of 200mg per kg body weight. After (25) days of treatment, mice were sacrificed. Hematoxylin and Eosin stains (H and E) were used to test the toxic effects of CPA on the liver and kidney histology. Our data showed the harmful effects of CPA drugs in the liver and kidney tissues, pathological changes were ranging from slight infiltration to sever necrosis and finally cytolysis also was observed. CPA-treated mice showed a significant (P<0.01) increased in serum creatinine, urea, and urea nitrogen levels. CPA-treated mice showed a tubular necrosis and inflammatory cell infiltration, as well as nephrotic changes when compared to control. CPA treatment demonstrated a significant (P<0.01) increased in Alanine Amino-Transferase (ALT), Aspartate Amino-Transferase (AST), Albumin and Bilirubin levels. Furthermore, livers of CPA-treated mice exhibited hepatocyte necrosis around central veins. Our data revealed the damaging and toxic effects of CPA against liver, kidney tissues and their biochemical functions.