Toxic effects of cadmium and lead exposure on intestinal histology, oxidative stress response, and microbial community of Pacific white shrimp Litopenaeus vannamei

Abstract

Cadmium (Cd) and lead (Pb) are two hazardous pollutants that threaten shrimp farming. The intestine is an important organ for digestion and immunity. We separately exposed Pacific white shrimp Litopenaeus vannamei to 500 μg/L Cd or 500 μg/L Pb seawater for 7 days, and 45 shrimp from each group were used to evaluate the changes of intestinal histopathological, oxidative stress, and microbiota composition. After Cd and Pb exposure, shrimp intestine appeared significant mucosal damage and oxidative stress, and the microbiota variation were induced. Specifically, the abundance of the phyla Bacteroidetes and Actinobacteria were induced, that of Proteobacteria and Firmicutes were deduced. The abundances of putative beneficial bacteria (Lactobacillus, Weissella, Demequina, Formosa and Ruegeria) and potentially pathogenic bacteria (Vibrio and Photobacterium) were fluctuated. Furthermore, the nutrient metabolic function of intestinal microbes was significantly altered. We concluded that Cd and Pb exposure had negative effects on the intestinal health of shrimp.