Abstract
Alachlor (lasso), like butachlor (machete) shows mutagenic properties when tested in Nostoc muscorum systems. The mutagenicity of the two herbicides seems considerably higher than that of MNNG ( N-methyl- N′-nitro- N-nhitrosoguanidine). A dose of alachlor considerably higher than that of butachlor is needed to obtain a comparable level of growth inhibition and mutagenesis, thus suggesting that butachlor is the more efficient mutagen. Methylamine-resistant (MA-R) and l-methionine- dl-sulfoximine-resistant (MSO-R) mutants have been obtained spontaneously or following mutagenic treatment with either herbicide. MA-R strains appear to metabolize methylamine as a nitrogen source. MSO-R strains do not form heterocysts in NO 3 − or NH 4 + medium containing or lacking MSO; the parent N. muscorum does not form heterocysts in NO 3 − or NH 4 + medium lacking MSO but does form them in either nitrogen medium containing MSO.
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