Toxic Activities of Platycladus orientalis Against Lasioderma serricorne and Tribolium castaneum in Stored Tobacco

Abstract

In order to effectively control the pests in stored tobacco leaves, new botanical protectants werestudied and developed. The activities of essential oil and isolated components of Platycladus orientalis weretested via fumigation and contact toxicity assays, and the chemical components in the essential oil wereanalyzed by GC/MS. The results showed that:1)A total of 34 components were identified in the essentialoil, which accounted for 93.28% of total components; and the major components were α-pinene(15.08%),cedrol(12.63%), 3-carene(9.68%)and caryophyllene(9.02%). 2)By means of bioactivity screening, theessential oil possessed potential contact toxicity against Lasioderma serricorne(LD50= 50.45 μg/adult)andTribolium castaneum(LD50 = 48.59 μg/adult), and it also possessed fumigation toxicity against Lasioderma serricorne(LC50=145.40 mg/L)and Tribolium castaneum(LC50=39.25 mg/L). 3)Three active components,α-pinene, β-phellandrene and 4-terpinenol, were obtained from the essential oil. It was proved that theessential oil of Platycladus orientalis and its active isolated components possessed certain contact andfumigation toxic activities against Lasioderma serricorne and Tribolium castaneum.