Towards upscaling the valorization of wheat straw residues: alkaline pretreatment using sodium hydroxide, enzymatic hydrolysis and biogas production.

Abstract

Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the development of anaerobic digestion technology. The physical and chemical characteristics of wheat straw (WS), as a representative lignocellulosic biomass, have a significant impact on the anaerobic digestion process in terms of quantity and quality of the produced biogas. This study aimed at investigating the enzymatic saccharification and detoxification of straw prior to anaerobic digestion with the final objective of enhancing the performance of conventional anaerobic systems of recalcitrant fractions of agricultural waste. The experimental activity was performed in lab and pilot scale treating WS. Alkaline delignification of straw using sodium hydroxide (NaOH) was studied prior to enzymatic hydrolysis for the production of easily biodegradable sugars. After defining the optimum conditions for the pretreatment scheme, the anaerobic digestability of the effluents produced was measured. Finally, the final liquid effluents were fed to a pilot scale anaerobic digester of 0.5m3 volume, applying an increasing organic loading rate (OLR) regime (in terms of chemical oxygen demand (COD) from 0.2 to 15kg COD/m3/day). The optimum conditions for the delignification and enzymatic hydrolysis of WS were defined as 0.5M NaOH at 50°C for 3-5h and 15μL Cellic CTec2/g pretreated straw at 50°C. It was proven that the resulting liquid effluents could be fed to an anaerobic digester in the ratio that they are produced with satisfactory COD removal efficiencies (over 70%) for OLRs up to 10kg COD/m3/day. This value is correspondent to a hydraulic retention time of around 7.5days, much lower than the respective one for untreated straw (over 12days).