Abstract
AbstractTriplex formation with double‐stranded DNA (dsDNA) by oligonucleotides has potential for applications in attractive technologies such as gene therapy and genetic diagnosis. However, triplex‐forming oligonucleotides (TFOs) can only recognize homopurine strands in homopurine‐homopyrimidine regions in dsDNA, either through Hoogsteen or through reverse‐Hoogsteen hydrogen bonds. A straightforward and powerful approach to overcoming this sequence limitation is the development of artificial nucleic acids capable of recognizing specific pyrimidine‐purine interruptions (i.e., a CG or TA base pair) in triplex formation. This review describes artificial nucleic acids, especially those containing non‐natural nucleobases, developed to recognize CG or TA base pairs in dsDNA targets.
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