Abstract
Sensitive and specific analysis of zearalenone (ZEN) mycotoxin in cereals for ensuring food safety is critical and remains challenging. Herein, a new gold nanoparticles @aptamer-functionalized hybrid affinity monolithic column was proposed and employed for online specific recognition of ZEN by HPLC. Characterization on the morphology, Brunauer–Emmett–Teller (BET) surface area mechanical stability and specific performance of the obtained affinity monolith were investigated. A super-high aptamer coverage density could reach 3636 pmol/μL, which is preferable to gain an effective analysis of ZEN with high specificity and a low interference of co-existed substances including typical α-Zearalenol (α-ZOL) and Aflatoxin B1 (AFB1). The sensitive recognition of trace ZEN was obtained with the limit of detection (LOD) as low as 0.05 ng/mL. Applied to real cereal samples, satisfactory recoveries were obtained in the range of 91.6 ± 1.4%–97.8 ± 2.6% (n = 3) in corn, 93.8 ± 3.1%–95.0 ± 3.6% (n = 3) in wheat, and 90.9 ± 4.7%–94.7 ± 3.8% (n = 3) in rice, respectively. The results on quantitative analysis were similar to that of LC–MS and better than that obtained by using immunoaffinity column (IAC) or molecularly imprinted polymer (MIP). This protocol provided an efficient access to high-efficient online specific recognition of ZEN in cereals by using such an aptamer-affinity capillary monolithic column.
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