Towards direct enzyme wiring: a theoretical investigation of charge carrier transfer mechanisms between glucose oxidase and organic semiconductors.

Abstract

In this work, a general theoretical and numerical approach based on semiconductor theory, which could be applied to a study of direct enzyme wiring, has been discussed. Marcus-Hush theory was applied to evaluate the potential transfer of charge carriers (holes and electrons) between glucose oxidase (GOx) and organic semiconductors. Two mechanisms of multistep hopping of charge to/from the oxidised/reduced flavin-based moiety through residues of aromatic amino acids located in GOx and long range charge direct tunnelling from the cofactor to the organic semiconductor surface have been proposed and evaluated. It was determined that the hole-hopping mechanism is possible and proceeds at a low ionization potential of the organic semiconductor. The calculations reveal that hopping of electrons is blocked, but direct electron tunnelling between the cofactor and the organic semiconductor is still probable. The most optimal conditions and tunable characteristics of GOx-based biosensors such as the ionization potential, electron affinity of organic semiconductors and distance between the enzyme and surface were estimated for the first time.