Abstract
Age-related non-enzymatic protein glycation leads in skin to the formation and subsequent accumulation of Advanced Glycation End products (AGEs), mainly carboxymethyl-lysine (CML) and pentosidine (1). Intracellular AGEs have been shown to accumulate in dermal fibroblasts upon aging, inducing oxidative stress and resulting in cell senescence and apoptosis (1, 2). The aim of this work was to use symbiotic microorganisms extracts to detoxify skin fibroblasts from AGEs. 80 extracts were screened on primary fibroblasts (54 years old donor). The intracellular glycation level was sufficiently high to allow the detection of global AGEs and pentosidine by measuring auto-fluorescence in cell lysates without additional glycation. Following 24h cell treatment the most active extracts decreased significantly the total amounts of AGEs (– 49%) and pentosidine (– 33%). The ability of 11 highly active extracts to reduce the intracellular amount of CML in fibroblasts was then tested using immunofluorescence technique. The 3 most active extracts decreased the amount of CML by 15% in addition to global AGEs and pentosidine. Phytochemical studies through bioguided fractionation showed increased activities of fractions (CML amount -27%). Transcriptional studies are also in progress to identify a biological mechanism of AGEs degradation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
