Towards biodegradable polyhydroxyalkanoate production from wood waste: Using volatile fatty acids as conversion medium

Abstract

Production of polyhydroxyalkanoate (PHA) via mixed microbial consortia is a potential economic alternative responding to the current demand for functional greener materials to replace traditional petroleum-basedpolymers. The goal of this study was to synthesize PHA using volatile fatty acids (VFAs) obtained from the co-fermentation of pretreated wood waste and sewage as carbon source. High PHA yield of 0.71 g COD PHA/g COD VFAs and PHA content of 50.3 g PHA/100 g VSS were obtained at VFAs ratio (even:odd) of 88:12 after seven cycles cultivation. Even acids were more suitable for accumulating PHA as the preferred carbon source than odd acids, resulting in 3-hydroxybutyrate being the main monomer. PHA production achieved to the highest value of about 2639 mg COD/L at 1400 mg COD/L VFAs concentration. The bacterial genera displayed a highly diverse of the microbial community for the synthesis of PHA.