Abstract
In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulose β-1–4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.
Highlights
Modulated endoglucanase activity allows for reduction of recalcitrance while maintaining normal plant growth
Expression in tobacco of a similar, but not hyper-thermophilic endoglucanase from Trichoderma reesei, TrCel5A, has been reported[25]
We suspect that if AcCel5A was expressed at high titers and fully active during early plant development, it too would likely result in undesirable phenotypes and altered chemical and physical properties of the cell wall polymers
Summary
Results AcCel5A expression in Arabidopsis reduces cell wall recalcitrance. The AcCel5A (n = 5) expressing plants have a 25% increase in glucan conversion compared to the empty vector (EV) control (n = 6) or the AcCel5A-D (n = 8) plants (Fig. 2c). AcCel5A expression in Arabidopsis does not impact growth, cell wall composition, or cellulose crystallinity.
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