Abstract
Abstract The Metalworking Fluids Project Group of the International Biodeterioration Research Group (IBRG) was formed in 1980 with one of its objectives being the development of standard methods for evaluating the inherent resistance of, and testing of antimicrobial compounds (biocides) in aqueous metalworking fluids. The work described below summarises work undertaken by the Group which has aided development of a preliminary screening test, entitled ‘A Screening Method for the Determination of Inherent Resistance to Biodeterioration of Aqueous Metalworking Fluids and for the Evaluation of Biocides in Metalworking Fluids (Test 1)’ (1992), recently published as a draft standard for comment by the Institute of Petroleum under the designation IP PM/BV 1994. The collaborative work reported here includes: 1. (i) Enumeration techniques for aerobic and anaerobic organisms in metalworking fluid emulsions. 2. (ii) The role of yeasts in the biodeterioration of metalworking fluids. 3. (iii) Investigation of a ‘clearing zone’ technique for determining biodeteriogenic species. 4. (iv) The influence of added organic nutrients. 5. (v) Determination of optimum growth temperatures for bacteria in metalworking fluids. 6. (vi) The development of a recommended inoculum. 7. (vii) The use of mycelial dry weight measurements to determine fungal growth.
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