Abstract
Several attempts to achieve the quantitative photoacoustic tomography (q-PAT) have been investigated using point sources or a single-angle wide-field illumination. However, these schemes normally suffer from low signal-to-noise ratio (SNR) or poor quantification in imaging applications on large-size domains, due to the limitation of ANSI-safety incidence and incompleteness in the data acquisition. We herein present a q-PAT implementation that uses multi-angle light-sheet illuminations and calibrated recovering-and-averaging iterations. The scheme can obtain more complete information on the intrinsic absorption from the multi-angle illumination mode, and collect SNR-boosted photoacoustic signals in the selected planes from the wide-field light-sheet excitation. Therefore, the sliced absorption maps over whole body of small-animals can be recovered in a measurement-flexible, noise-robust and computation-economic way. The proposed approach is validated by phantom, ex vivo and in vivo experiments, exhibiting promising performances in image fidelity and quantitative accuracy for practical applications.
Highlights
Photoacoustic tomography (PAT) has the unique capabilities to provide high optical absorption contrast and excellent spatial resolution for soft tissue imaging [1,2,3,4,5]
We introduce a calibrated 2-D reconstruction scheme proposed by Schweiger and Arridge, which has been widely used in diffuse optical tomography (DOT) reconstruction [29,30,31,32]
Output: the distribution of the absorption coefficients μa It is worth noting that the regularization parameter σ here is set to ensure long-term convergence of the algorithm, and the same finite element method (FEM) mesh is adopted for the reconstruction of each incidence angle
Summary
Photoacoustic tomography (PAT) has the unique capabilities to provide high optical absorption contrast and excellent spatial resolution for soft tissue imaging [1,2,3,4,5]. To quantitatively map the distribution of local optical absorption coefficients from the cPAT results and enhance the applicability of q-PAT in more practical cases, we develop a q-PAT imaging implementation combining the wide-field light-sheet excitation and multi-angle illumination mode. The former enables boosting SNR of P0 images from the specific-size light-sheet illumination and guarantees the excitation in a certain imaging plane without loss of modelling simplicity of the photon fluence. The ex vivo and in vivo experiments demonstrate the improvement of image fidelity and recovery of the optical absorption coefficient in practical applications compared to cPAT
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