Toward using Margaritifera falcata as an indicator of base level nitrogen and carbon isotope ratios: insights from two California Coast Range rivers

Abstract

Measurements of freshwater mussel tissue are potentially very useful for determining base-level isotopic values for food web studies in aquatic environments. As long-lived, filter-feeding organisms, mussels have the potential to spatially and temporally average the isotopic baseline signal. Following from earlier studies that focused on lake environments, this study investigates the stable carbon and nitrogen isotope ratios in tissue of the river dwelling freshwater mussel, Margaritifera falcata, in two extensively studied northern California coast range rivers, the South Fork Eel and Navarro. We highlight advantages and challenges for using riverine mussel isotopes as indicators of baselines. δ13C of primary producers is known to vary with habitat along the South Fork Eel channel, but our measurements show no such variations, demonstrating that riverine mussels do preserve a spatially averaged measure of instream derived food sources. Mean δ13C and δ15N are shown to be markedly different in the two rivers, reflecting differences in food sources and possibly watershed land use. We also found that δ15N of mussel tissue increased by approximately 2‰ with mussel age in both rivers. This suggests it is important to consider age and size effects when estimating baseline values from mussel tissues.