Abstract
For some years laboratories working with ancient DNA have been optimising extraction methods using either undamaged modern DNA or authentic ancient DNA. This approach is unsatisfactory for a number of reasons, chief being the inherent variability from sample to sample. In addition, quantitative comparison of methods is generally impossible using typically small samples of ancient DNA, as well as being ethically questionable. We have now perfected a method whereby we can oxidatively damage the plasmid pUC19 using copper sulfate, ascorbic acid and hydrogen peroxide to create artificially damaged DNA that mimics the behaviour of ancient DNA. We have used this damaged plasmid to assay our extraction methods to quantitatively monitor the Archaeological science under a microscope: studies in residue and ancient DNA analysis in honour of Thomas H. Loy Watson, Connell, Harding and Whitchurch: TOWARD USING AN OXIDATIVELY DAMAGED PLASMID AS AN INTRAAND INTERLABORATORY STANDARD IN ANCIENT DNA STUDIES
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