Toward stereovisual monitoring of three-dimensional translations with submicrometer resolution

Abstract

We reported on stereovisual localization of a labeled target versus three-dimensional (3D) position and orientation with a resolution of a few micrometers [Opt. Express 18, 24152 (2010)]. A pseudo-periodic pattern (PPP) is fixed on the target, whose center is identified with subpixel accuracy in both stereo images. This subpixel position definition is fed into the geometrical model of the stereovision system and, thus, leads to subvoxel resolution in the 3D target positioning. This paper reports on improvements and specialization of the method for addressing the measurement of 3D translations: (a) The use of an encrypted PPP wider than the field of observation of the cameras has two beneficial effects. First, the allowed lateral target displacements are wider than the field of view of each camera, thus extending the workspace volume. Second, the 3D position is always derived from the same zone located at the center of the camera sensor chip. A simplified geometrical model is thus sufficient, and the effects of the lens distortions lead to a different kind of calibration issues. (b) By considering only translations, the pattern directions remain stationary in the recorded images. Two-dimensional Fourier transforms are then replaced by single dimension ones, thus reducing the computation time. (c) The choice of a higher magnification lens allows the achievement of submicrometer resolution in target position determination. This level of performance makes the method attractive in various automated applications requiring microstage position control and sensing. This approach may, for instance, fulfill the requirements for the coarse positioning of specimens in front of nanotechnology instruments that are equipped with their own high-accuracy but short-excursion-range translation stages.