Toward potential applications of the nitrogen nutrition index in Sweet basil (Ocimum basilicum L.) production under greenhouse conditions

Abstract

The nitrogen nutrition index (NNI) as an effective indicator for monitoring crop nitrogen (N) status has multiple applications in agriculture, such as estimating in-season crop N requirement (NR), predicting crop biomass/yield, and improving N use efficiency. The present study was aimed to develop basil (Ocimum basilicum L.) NR and relative biomass (RB) prediction models based on the concept of critical N concentration. Two experiments were carried out with different N application rates in the research greenhouse of University of Tehran, Iran. Basil NNI values were calculated using the previously developed critical N dilution curve. Subsequently, the in-season basil NR estimation model was developed as a function of NNI, N recovery efficiency (NRE) and plant age. Additionally, basil RB was simulated as a function of the averaged and integrated NNI based on a linear-plateau model. Moreover, a theoretical relationship between N utilization efficiency (NUtE) and the NNI was established. The validation results of the developed NR-NNI model demonstrated a good performance with a root mean square error (RMSE) and a normalized root mean square error (NRMSE) less than 5.28 kg ha−1 and 4.72%, respectively. The results showed that crop biomass and NNI were the most significant explanatory factors for the variations in basil NUtE. Therefore, the crop NNI can serve as a practical in-season diagnostic tool for basil NR and RB prediction, while also contributing to the design of decision support systems and sustainable agro-ecosystems.