Toward a Complete North American Borrelia miyamotoi Genome.

Luke C Kingry,Neeta Connally,Jeannine M Petersen,Lori A Rowe,Marc Dolan,Dhwani Batra,Christopher Sexton,Martin E Schriefer,Adam Replogle

doi:10.1128/genomea.01557-16

Luke C Kingry, Neeta Connally + Show 7 more

Open Access

https://doi.org/10.1128/genomea.01557-16

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Abstract

ABSTRACTBorrelia miyamotoi, of the relapsing-fever spirochete group, is an emerging tick-borne pathogen causing human illness in the northern hemisphere. Here, we present the chromosome, eight extrachromosomal linear plasmids, and a draft sequence for five circular and one linear plasmid of a Borrelia miyamotoi strain isolated from an Ixodes sp. tick from Connecticut, USA.

Highlights

Borrelia miyamotoi, of the relapsing-fever spirochete group, is an emerging tick-borne pathogen causing human illness in the northern hemisphere
The B. miyamotoi genome consists of a large linear chromosome (~1 Mb) and an unknown number of extrachromosomal linear and circular plasmids [7]
Pure culture of B. miyamotoi CT13-2396 was achieved from a single Ixodes scapularis nymph reared from the egg clutch of an infected adult female collected in 2013 from Connecticut, USA

Summary

Introduction

Of the relapsing-fever spirochete group, is an emerging tick-borne pathogen causing human illness in the northern hemisphere. The B. miyamotoi genome consists of a large linear chromosome (~1 Mb) and an unknown number of extrachromosomal linear and circular plasmids [7]. Sequence data for B. miyamotoi plasmids currently exist in unfinished raw contig form. The assembly of plasmids is complicated by the presence of long repetitive and paralogous sequences [8, 9].

Results

Conclusion