Total Polyphenols, Total Flavonoids, Antioxidant Activity and Inhibition of Tyrosinase Enzymes from Extract and Fraction of Passiflora ligularis Juss

Abstract

Background: Sweet granadilla (Passiflora ligularis Juss) grows in the cool highlands of Indonesia, one of which is the province of West Sumatera. Sweet granadilla has potent antioxidant activity and can inhibit the tyrosinase enzyme. Objective: This study was performed to determine content of total polyphenols, total flavonoids, antioxidant activity, tyrosinase inhibition in different part of P. ligularis extract and fraction. Materials and Methods: Leaves, stems, peels and seeds P. ligularis were separately extracted by the ultrasound-assisted extraction (UAE) method using 70% ethanol. Then, the ethanol extract was fractionated using n-hexane, ethyl acetate and distilled water. The ethanol extract and active fraction were determining antioxidant activity using FRAP and DPPH method, inhibition of tyrosinase enzyme, total polyphenol and total flavonoid content. This study was equipped with analysis of light microscopy, SEM microscopy and LC-MS. Results: The highest total polyphenol content was found in the seed extract 176.22 ± 1.51 mg GAE/g extract and total flavonoid content was found in leaves extract 5.77 ± 0.48 mg QE/g extract. The highest antioxidant activity by FRAP method was found in seeds extract 80.79 ± 1.29 g Fe2SO4 equivalent/100 g extract and DPPH method was found in stem extract with IC50 value 9.00 ± 0.09. The highest percentage of tyrosinase inhibition (1 mg/ml) was found in seed extract 52.4 ± 2,55 %. In fraction of seed extract show that ethyl acetate fraction most active than others. Conclusion: These results indicate that ethyl acetate fraction of seed P. ligularis has potent antioxidants and good inhibition of the tyrosinase enzyme.