Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde

Abstract

Mushrooms have been used as food and medicinal purposes for centuries. In this study, some edible Ascomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the samples were performed afterward. PCR products were sent for sequence analysis after ITS fragments were amplified with suitable primers concerning DNA isolation of samples. The obtained data were compared with the Genbank database for samples’ determination. The mushroom samples were determined to be Terfezia claveryi Chatin and Morchella esculenta (L.) Pers. Samples were extracted by ethanol and methanol to determine antimicrobial activity using the disc diffusion method. Antimicrobial zones against microorganisms were measured. Then DPPH radical scavenging effects and total phenolic content of T. claveryi and M. esculenta mushroom samples were studied. The highest antimicrobial activity was observed in ethanol extracts of M. esculenta. When the DPPH radical scavenging activities are taken into account, the activity is increased depending on the concentration. The highest DPPH scavenging activity was found in the extract of M. esculenta with 40.86%. It was determined that the total phenolic substances in methanol extracts of mushroom samples varies between 533.28 ± 1,15-537.34 ±2.20 μg GAE / mg extract.