Total phenolic content, total flavonoid content, and antioxidant activity of water and ethanol extract from Surian (Toona sinensis) leaves

Abstract

Surian (Toona sinensis) is a forest plant that is widespread in Asia. In Indonesia, this plant only used and known as a commodity for carpentry but in other nation, saurian is known as spices and traditional medicine because of its natural antioxidant potential. The objective of this study was assayed total phenolic content, total flavonoids content, and antioxidant activity from Surian leaves. Surian leaves were extracted by water and ethanol solvent. Water extraction was conducted by reflux in 90 °C then ethanol extraction was conducted by maceration, which is through various concentration 70%, 80%, 90%, and 96% of ethanol. Total phenolic content varied from 276.62 to 444.68 (mg GAE/g) and total flavonoid content ranged from 209.23 to 324.61 (mg QE/g). Antioxidant activity of the water and ethanol extracts was evaluated using DPPH (1,1-diphenyl-2-picryl-hydrazil) radical scavenging method. Total phenolic and flavonoid content of ethanol 90% extract and ethanol 96% extract had a higher amount than the other. The ethanol 96% extract had the strongest DPPH inhibition concentration 50% (IC50), which is 3.38 μg/mL while ascorbic acid is 3.90 μg/mL as a comparison. Antioxidant activity had a strong correlation with total flavonoid content.