Total Phenolic Content and Antioxidant and Antibacterial Activities of Pereskia bleo.

Mas Athira Johari,Heng Yen Khong

doi:10.1155/2019/7428593

Mas Athira Johari, Heng Yen Khong

Open Access

https://doi.org/10.1155/2019/7428593

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Abstract

Different solvent extracts of Pereskia bleo leaves were evaluated for total phenolic content (TPC) and antioxidant activities based on the Folin–Ciocalteu test and DPPH scavenging activities. The antibacterial activities against four bacteria, namely, Gram-positive bacteria: Streptococcus pyogenes ATCC 19615 (SP) and Staphylococcus aureus ATCC 29737 (SA) and Gram-negative bacteria: Escherichia coli ATCC 10536 (EC) and Pseudomonas aeruginosa ATCC 9027 (PA), were also performed based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. The findings demonstrated that both the methanolic and chloroform extracts displayed strong activities against SA, SP, EC, and PA while the hexane extract demonstrated the weakest activities towards all the four bacteria. The methanolic extract also exhibited higher TPC and possessed higher antioxidant activity with the IC50 value 33.83 µg/mL compared to the chloroform and hexane extracts. As such, the methanolic extract has a higher ability to scavenge free radical compared to other extracts. Due to the interesting result, activities are shown by the methanolic and chloroform crude extracts of P. bleo; hence, the study has been extended to the isolation of bioactive compounds to uncover its great potential as a natural source for antibacterial and antioxidant agents.

Highlights

Medicinal plants have been used to promote human health, and it has been documented since ancient times
P. bleo is well known as the medicinal plant of the Cactaceae family, which consists of 100 genera and about 2000 species [1]. e origin of the genus Pereskia is from South America, and they have been cultivated in tropical countries [2] such as Malaysia, Indonesia, Singapore, and
Phenolic compounds that contained in the plants have redox properties, and the properties allow them acting as antioxidants [6, 7]. e results (Table 1) showed that the methanolic extract exhibited higher total phenolic content (TPC) as compared to the chloroform and hexane extracts which are approximately about 40.82 mg GAE/g for methanolic extract, 31.91 mg GAE/g for chloroform extract, and 25.2 mg GAE/g for hexane extract

Summary

Introduction

Medicinal plants have been used to promote human health, and it has been documented since ancient times. Higher phenolic content in the methanolic extract is responsible for bioactivity; this extract is expected to exhibit good result in antioxidant and antibacterial activities. E results (Figure 1) showed percentage inhibition of DPPH radical of the Pereskia bleo crude extract and standard at different concentrations.

Results

Conclusion