Abstract
A comparative study was carried out with ethanolic (80%) extracts from leaves and cell cultures of three Callistemon species, namely C. lanceolatus (CL), C. viridiflorous (CV), and C. comboynensis (CC). Cell suspensions of the three species were grown in liquid Murashige and Skoog (MS) medium (100 ml) supplemented with 0.9 mg·g-1 kinetin in combination with 1.1 mg·g-1 NAA. The CL leaf extract was standardized to contain the highest amount of phenolics (104 ± 2.0 mg·g-1), followed by CC (95.8 ± 1.2 mg·g-1) and CV (79.8 ± 4.6 mg·g-1). On the other hand, cell cultures of CV contained more phenolics (14.9 ± 0.6 mg·g-1) than those of the other two species, CL and CC, which contained 12.2 ± 0.16 and 9.12 ± 0.16 mg·g-1, respectively. Nevertheless, CV leaf extract exhibited the highest antioxidant activity (91.4% ± 0.4%) at a concentration of 1000 µg·ml-1, comparable to 100 µg·ml-1 gallic acid (90.8% ± 1.5%).
Highlights
The genus Callistemon (Myrtaceae) contains 34 species of beautiful evergreen shrubs and small trees
A comparative study was carried out with ethanolic (80%) extracts from leaves and cell cultures of three Callistemon species, namely C. lanceolatus (CL), C. viridiflorous (CV), and C. comboynensis (CC)
T is the total phenolic content in mg·g–1 of the extracts as gallic acid equivalent (GAE), C is the concentration of gallic acid established from the calibration curve in mg·ml–1, V is the volume of the extract solution in ml and M is the weight of the extract in g
Summary
The genus Callistemon (Myrtaceae) contains 34 species of beautiful evergreen shrubs and small trees. The majority of the Callistemon species is endemic to the more temperate regions of Australia, four species are found in New Caledonia and seven species have been introduced to India as ornamental trees [1] They are commonly known as bottle brushes because of their cylindrical brushlike flowers resembling the traditional bottle brush. In vitro cultures have the potential to form secondary metabolites and to exhibit bioactivity comparable to the original plant [4,5]. Phenolic compounds exhibit considerable free-radical scavenging activity, which is determined by their reactivity as hydrogen- or electron- donating agents, their reactivity with other antioxidants and their metal chelating properties, as well as the stability of the resulting antioxidant-derived radicals [10,11]. Three Callistemon Species cell cultures of three Callistemon species with respect to their potential as antioxidant agents in relation to their total content of phenolic compounds
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