Abstract

Total phenol levels and antioxidant activity in whole limes from Palopo, Luwu Regency, as well as the relationship between total phenol content and antioxidant activity, were determined. Maceration of dried whole lime fruit yielded the methanol extract (Citrus aurentifolia). Phytochemical screening was used to determine the presence of alkaloids, phenolic compounds, and flavonoids in the extract’s secondary metabolites. The total phenol extract was determined using the Folin-Ciocalteu method, yielding a value of 8.37 mgGAE/g. Antioxidant activity was determined using the DPPH method, and the IC50 value was 1793.06 g/mL. Total phenol content of whole fruit extract lime and IC50 show a linear relationship y = -168,11x + 3211 with a determination R2 = of 0.9780.

Highlights

  • Because the human body only has a limited number of antioxidants, if there are a lot of radicals in the body, the body requires antioxidants from outside [1]

  • The use of natural resources as a source of natural antioxidants is preferable because it produces fewer side effects than synthetic drugs, which frequently produce side effects that are more harmful to health

  • This study was focused on knowing the total phenolic content and antioxidant activity of the methanol extract contained in the whole fruit of lime (Citrus aurentifolia) and the relationship between total phenolic and its antioxidant activity

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Summary

Introduction

Because the human body only has a limited number of antioxidants, if there are a lot of radicals in the body, the body requires antioxidants from outside [1]. Investigation in this research It has never been done before, we determine the total phenol content and antioxidant activity of whole limes without separating the skin and juice. The public's use of lime fruit for extracting juice is based solely on experience and educated guesses. Antioxidant compounds such as flavonoids, tannins, anthraquinones, cinnamates, and xanthones, have been proven to be used as immune enhancers [9,10]. This study was focused on knowing the total phenolic content and antioxidant activity of the methanol extract contained in the whole fruit of lime (Citrus aurentifolia) and the relationship between total phenolic and its antioxidant activity

Tools and materials
Maceration and extraction
Identification
Alkaloid test
Flavonoid test
Steroids and triterpenoids test
Saponin test
Phenolic test
Determination of the maximum wavelength of gallic acid
Making gallic acid calibration curve with Folin-Ciocalteu reagent
Determination of total phenol content by Folin-Ciocalteu method
Analysis of Total Phenolic
Saponin
Flavanoid test
Analysis of total phenol levels
Antioxidant activity test antioxidant
Conclusion

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