Total petroleum hydrocarbons and influencing factors in co-composting of rural sewage sludge and organic solid wastes

Abstract

Co-composting is an efficient strategy for collaborative disposal of multiple organic wastes in rural areas. In this study, we explored the co-composting of rural sewage sludge and other organic solid wastes (corn stalks and kitchen waste), with a focus on the variation of total petroleum hydrocarbons (TPH) during this process. 12% corn-derived biochar was applied in the composting (BC), with no additives applied as the control treatment (CK). The TPH contents of piles after composting ranged from 0.70 to 0.74 mg/g, with overall removal efficiencies of 35.6% and 61.1% for CK and BC, respectively. The results indicate that the addition of 12% biochar increased the rate of TPH degradation and accelerated the degradation process. 16s rDNA high-throughput sequencing was applied to investigate the biodiversity and bacterial community succession during the composting process. Diverse bacterial communities with TPH degradation functions were observed in the composting process, including Acinetobacter, Flavobacterium, Paenibacillus, Pseudomonas, and Bacillus spp. These functional bacteria synergistically degraded TPH, with cooperative behavior dominating during composting. Biochar amendment enhanced the microbial activity and effectively promoted the biodegradation of TPH. The physicochemical properties of the compost piles, including environmental factors (pH and temperature), nutrients (nitrogen, phosphorus, potassium), and humic substances produced in composting (humic acids and fulvic acids), directly and indirectly affected the variation in TPH contents. In conclusion, this work illustrates the variation in TPH content and associated influencing factors during co-composting of rural organic solid wastes, providing valuable guidance toward the further optimization of rural organic waste management.