Abstract
One of marine resources that has high value is seaweed. Seaweed is a carrageenan producer used in the food industry. Seaweed contains many minerals, vitamins and proteins that are useful for health. Carotenoids are pigments found in seaweed that function as antioxidants. The genes involved in carotenoid biosynthesis have been studied and provide opportunities for genetic improvement of seaweed. DNA is a basic requirement in molecular analysis. Therefore, a suitable method of DNA extraction from seaweed is needed. The aim of this research was to investigate DNA extraction method from several seaweed species and test the DNA quality through PCR-RAPD. Seaweed samples were collected from Pantai Bumi Perkemahan Taman Nasional Bali Barat and DNA was extracted using Doyle and Doyle’s method with modifications. PCR-RAPD was conducted using primer UBC127 and OPD 11 to test the quality of DNA. Results showed that 3 hours incubation in 60ºC had the best result of DNA extraction. However, the quality of DNA was low, as indicated by inconsistent PCR-RAPD products. Further optimization in DNA extraction is needed to obtain high quality DNA for genetic analysis.
Highlights
Bali has a variety of coastal and marine ecosystems such as mangroves, corals and seaweed that have important values such as for food and medicine
The genes involved in carotenoid biosynthesis have been studied and provide opportunities for genetic improvement of seaweed
PCRRAPD was conducted using primer UBC127 and OPD 11 to test the quality of DNA
Summary
Bali has a variety of coastal and marine ecosystems such as mangroves, corals and seaweed that have important values such as for food and medicine. Development of marine resources into a source of medicines, is important for both the development of science and economic. Market demand for natural ingredients as a source of food and medicine is increasing rapidly [1]. One of the ingredients that is currently very popular in the market is carotenoids. Carotenoids are fat-soluble pigments, which are a group of yellow, orange, red and brown pigments found in various organisms such as: phytoplankton, seaweed, bacteria and certain plants. Humans and animals consume carotenoids to meet the needs in their bodies as antioxidants and are very dependent on external supplies [2]
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