Abstract
Metastasis is the major cause of cancer-related deaths. Targeting the process of metastasis has been proposed as a strategy to fight cancer. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is used for treatment of many diseases, including cancer. This study aimed to determine the anti-metastatic effect of total flavonoids of S. barbata (TF-SB) using the human hepatocarcinoma MHCC97H cell line with high metastatic potential. Our results show that TF-SB could significantly inhibit the proliferation and invasion of MHCC97H cells in a dose-dependent manner. MMP-2 and MMP-9 expression were obviously decreased after TF-SB treatment at both the mRNA and protein level. TIMP-1 and TIMP-2 expression were simultaneously increased. The present study indicates that TF-SB could reduce the metastatic capability of MHCC97H cell, probably through decrease of the MMP expression, and simultaneous increase of the TIMP expression.
Highlights
Hepatocarcinoma is one of the most common cancers, with high incidence and mortality
We investigated the anti-metastatic effect of total flavonoids of S. barbata (TF-Scutellaria barbata D. Don (SB)) using the human hepatocarcinoma MHCC97H cell line to clarify the possible molecular mechanism of TF-SB in inhibiting cancer cell invasion
MHCC97H cells were treated with different doses of TF-SB
Summary
Hepatocarcinoma is one of the most common cancers, with high incidence and mortality. Metastasis is a multi-step process, including local invasion, lymphatic and blood vascular system, survival in the bloodstream, extravascularizations from the microvessels and colonization at the secondary site [5,6]. This process depends on the activities of many factors associated with the proteolytic degradation of extracellular matrix (ECM) components [7]. We investigated the anti-metastatic effect of total flavonoids of S. barbata (TF-SB) using the human hepatocarcinoma MHCC97H cell line to clarify the possible molecular mechanism of TF-SB in inhibiting cancer cell invasion
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