Abstract
Six samples collected at different stages during the separation of total flavonoids from crude extracts of the fresh stems and leaves of Gynura bicolor DC. were analyzed for total flavonoid contents. After undergoing acid hydrolysis, the crude extracts were determined for quercetin and kaempferol by high-performance liquid chromatography(HPLC). The amount of total flavonoids was calculated based on the measured flavonoid compounds. After sequential column chromatographies on macroporous resin and polyamide, the total flavonoid content of purified products was 84.8%, and further recrystallization formed two products containing 97.1% and 99.7% total flavoniods, respectively. In conclusion, the process involving ethanol extraction, separation by sequential column chromatographies on macroporous resin and polyamide and recrystallization offers an effective way to purify total flavonoids from the fresh stems and leaves of Gynura bicolor DC.
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