Abstract
According to the online software, the primer is designed by CODEHOPE(Consensus De-generate Hybrid Oligonucleotide Primers),then the cDNA sequence of Chalcone synthase was obtained by the method of RACE(GenBank No. HQ434624).Then the high efficiency vector named pCM-BCHS was constructed by pCAMBIA1301 and Chalcone synthase gene. The vector was transformed byAgrobacterium tumefaciensto buckwheat induced callus cell. At last the selected transgene callus cell can synthase the total flavonoid at 48.3mg/g DW, which is two times of the wild type. Therefore, it is proved that the overexpression of Chalcone synthase will enhance the metablic flux of total flavonoid synthesis.
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