Abstract
Knowing the total-body water content of their patients, physicians can reach important conclusions. So far, complex methodology has been an obstacle to the easy obtainment of precise data. The presented method with scintillation counting, using an internal standard employs two identical vials with equal quenching. Both of the vials contain the same amount of sample, but only one of them a known amount of HTO. With the proposed mathematical handling of the readings the efficiency can be determined and with the concentration of HTO in the samples, the HTO diffusible space. To test the procedure urine and serum samples taken 24 hr after oral HTO served to determine the total-body water. The results for men were: 649 per cent of the body weight or 239 1/m2 of the body surface; women: 56·6 per cent of the body weight or 19·71/m 2 of body surface. The perfect reproducibility, accuracy and simplicity of the procedure offer an excellent and practical method to determine total body water.
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