Total area of C-heterochromatin in nuclei and chromosomes as a measure of cultivated flax genome variability

Abstract

Comparative statistical analysis of total C-heterochromatin content (total area of darkly stained C-bands) in prophase nuclei and metaphase chromosomes of eight flax (Linum usitatissimum L.) varieties, viz., three oil flax varieties (Shafir, Oliver, Linotte), three modern (Orshansky-2, Belinka, Baltuchai) and two ancient fiber flax varieties (Zaretsky Kryazh, K-37) has been carried out. Karyotypes of flax varieties selected for different agronomical traits differed from one another in C-heterochromatin content. The total C-band area of oil flax varieties exceeded that of fiber flax varieties. Genomic polymorphism in C-heterochromatin markers is a testimony of different breeding directions. A simple test for determining total C-heterochromatin content in prophase nuclei is recommended as a useful tool for screening specimens with predetermined characteristics during selection of new varieties.