Total antioxidant capacity and protein peroxidation intensity in seminal plasma of infertile and fertile dogs.

Abstract

The aim of this study was to evaluate the total antioxidant capacity and protein peroxidation intensity in seminal plasma of infertile and fertile dogs. The study was conducted on 10 infertile and 10 fertile dogs of various breeds. Infertility was defined as conception failure at least three matings with different bitches. Semen was collected by manual manipulation. The sperm concentration and motility parameters were evaluated using CASA Hamilton Thorne, Vers. IVOS 12.3. The morphology of spermatozoa and the percentage of live and dead sperm cells were assessed microscopically, total antioxidant capacity and the content of SH-groups in seminal plasma were determined spectrophotometrically, the contents of protein peroxidation markers in seminal plasma, bityrosine and formylokinurenine, were determined using spectrofluorimetric methods. Sperm concentration and total sperm count were significantly (p<0.05) lower in infertile dogs than in fertile dogs (99.92±3 0.05×106 /ml vs. 282.07±48.27×106 /ml; 214.19±114.74×106 vs. 747.57±210.94×106 , respectively). The percentage of spermatozoa with normal morphology and the most determined motility parameters differed significantly (p<0.05) between both groups. The mean values of total antioxidant capacity in the seminal plasma were significantly (p<0.05) lower (19.95±20.94 vs. 25.66±23.18µmol/g protein), whereas the mean contents of bityrosine and formylokinurenine in seminal plasma were significantly (p<0.05) higher in infertile dogs than in fertile dogs (3.71±4.83µg/mg protein vs. 1.55±2.00µg/mg protein and 0.37±0.45µg/mg protein vs. 0.14±0.08µg/mg protein, respectively). In conclusion, the obtained results suggest that the poor semen quality and infertility in dogs could be associated with lowered total antioxidant capacity and increased protein peroxidation in seminal plasma as a consequence of oxidative stress.