Total and differential leucocyte counts in rat and mouse bronchoalveolar lavage fluids using the Sysmex XT-2000iV

Abstract

The introduction of the Sysmex XT-2000iV analyser now provides a haematology analyser with five-population differential leucocyte counting for several laboratory animal species. In addition, there are options for counting total leucocytes in rat and mouse bronchoalveolar lavage fluids (BALF). In these studies, total leucocyte counts were made using the Sysmex XT-2000iV, the Sysmex XT-2000i and the Bayer Advia 120™. The results were found to be comparable for rat and mouse BALF and mouse air pouch lavage fluids. Imprecision, sample carry-over, linearity and stability data were also studied. In addition, results were obtained for the percentage cell counts of eosinophils and neutrophils, and these data were compared with manual 200 cell counts using a cytospin technique. In summary, the Sysmex XT-2000iV proved to be suitable for BALF total leucocyte, neutrophilia and eosinophil counting.