Total and Differential Cells of Leukocyte Mice (Mus musculus) On Evaluation In Vivo Anticancer Extracts Ethanol Marine Sponges Aaptos suberitoides

Abstract

The aim of this research is to know activity of ethanol extract from marine spons Aaptos suberitoides in total number and differentiation leukocyte cells which induce by carcinogenic agent Benzo(a)pyrene. METHODS. Mice (Mus musculus) were grouped in 6 group, group I (healthy mice), group II (induce by CMC Na), group III (treatment by cyvlophospamide), group IV (administered by spons extract 500 mg/kg BW), group V (administered by spons extract 1000 mg/kg BW) and group VI (administered by spons extract 1500 mg/kg BW). Benzo(a)pyrene induced for 10 days (5 times). The doses that given is 0,3 gram / 0,2 ml CMC Na. Mice were killed and the blood were taken. The sacrificed and blood sampling were taken. The total number and differentiation of leukocyte cells is made by smear method. RESULT AND DISCUSSION. The result of this examination is there were average total number of leukocyte cells in group I was 10,800 cells/μl, II and VI group were 3,700-5,500 cells/μl, the groups III, IV, V of 2,100-5,500 cells μl. The number of neutrophils and lymphocytes showed a significant difference (P <0.05) while the monocyte was no significant difference (P> 0.05).