Abstract
A scanning ion conductance microscopy (SICM) can image living samples in liquid by detecting ion current through a nanopipette. The nanopipette has an aperture of approximately 100 nm in diameter. In the SICM technique, it is possible to position the nanopipette edge in the vicinity of the sample surface by detecting the reduction of the ion current. By the way, most biological samples have electrostatic charge on the sample surface in liquid, which influences the ion current detected by the SICM. Thus, it is difficult to observe such charged samples without artifacts and noise generated by the charge effect. In this paper, to observe charged samples, we used a theta nanopipette as a probe of the SICM. Furthermore, we additionally developed a method for obtaining a charge map of the sample surface. By using the novel method, a topographical image and a charge map of the charged sample surface were successfully obtained simultaneously.
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