Topikal Uygulamalar için Antimikrobiyal Kitosan-Sodyum tetrafloroborat (NaBF4) Hidrojeller

Abstract

Infection of a wound is one of the most important reasons delaying the recovery of an injured tissue. In this study, chitosan-based hydrogels were loaded with different concentrations of sodium tetrafluoroborate (NaBF4) to fabricate an antimicrobial wound care system. Antimicrobial activity, and cytotoxicity of NaBF4, and surface morphology, chemical bond structures and antimicrobial activity of Chitosan:NaBF4 hydrogels against a broad spectrum of microorganisms including an antibiotic resistant species were investigated. NaBF4 showed higher antibacterial activity for gram-positive bacteria than gram-negative bacteria. MIC values of NaBF4 were 3.906, 1.953, and 7.813 µg/µL for every gram-negative, gram-positive, and fungal species, respectively. Direct cytotoxicity of NaBF4 on the L929 cell line was investigated by the MTT assay. IC50 value after 24 h incubation was calculated as 3.2 µg/µL which is within the range of concentration with antimicrobial activity. The antimicrobial activities of chitosan hydrogels were investigated by disc diffusion method. Antimicrobial activity of hydrogel increased with increasing NaBF4 concentration while parent high molecular weight chitosan-based hydrogel did not show antimicrobial activity. According to the results, sample 1:3 (NaBF4 concertation is 546.5mM in the hydrogel) was enough to achieve broad spectrum antimicrobial activity and hydrogels prepared with this formulation can be used as a potential antimicrobial wound care product.