Topical Hypericum perforatum Improves Tissue Regeneration in Full-Thickness Excisional Wounds in Diabetic Rat Model.

Soheila Yadollah-Damavandi,Yekta Parsa,Mehdi Chavoshi-Nejad,Ehsan Jangholi,Noushin Nekouyian,Maryam Mohsenikia,Amin Seifaee,Shima Rafiee,Sahar Hosseini,Soheil Ashkani-Esfahani,Hossein Karimi

doi:10.1155/2015/245328

Abstract

Delayed wound healing process is one of the most important concerns in diabetes. Healing of wounds has four phases, namely, hemostasis, inflammation, proliferation, and remodeling. For a successful repair, all four factors must occur properly. Hence, we aimed to evaluate the healing effects of Hypericum perforatum (HP) on full-thickness diabetic skin wounds by using stereological methods. Forty-eight female diabetic rats were randomly divided into four groups (n = 12): gel base treated group, HP 5% gel treated group, HP 10% gel treated group, and the control group which received no treatment. A circular 1 cm2 full-thickness wound was created on the animal's neck and wound area was measured every three days. After sacrificing the animals, skin samples were fixed and prepared for stereological evaluations. Based on the results, HP treated group showed faster wound closure rate in comparison with control and vehicle groups (P < 0.05). In addition, numerical density of fibroblasts, volume density of collagen bundles, and mean diameter and volume densities of the vessels in HP group were significantly higher than control and vehicle groups. The results of this study showed that HP has the ability to improve tissue regeneration by enhancing fibroblast proliferation, collagen bundle synthesis, and revascularization.

Highlights

It is reported that there are more than 347 million people with diabetes all over the world [1]
In order to facilitate the application of the agent, we provided 5% and 10% Hypericum perforatum (HP) gel by dissolving 5 cc (HP 5) and 10 cc (HP 10) of the extract each in 2 cc distilled water and transferred the solution into 2% carboxymethylcellulose (CMC) (2 g CMC dissolved in 98 cc distilled water) [13]
The populations of the fibroblasts in the HP 5% and HP 10% treated groups were 59.61% (P = 0.023) and 51.07% (P = 0.034) higher than the controls, respectively, and 96.49% (P = 0.009) and 85.98% (P = 0.013) higher than the gel base group, respectively (Table 1)

Summary

Introduction

It is reported that there are more than 347 million people with diabetes all over the world [1]. One of the most important concerns of diabetic patients is delayed wound healing which can lead to serious complications such as limb amputations, organ dysfunctions, cosmetic impairments, infections, sepsis, and even death [2]. Diabetic patients suffer from impaired vascularization, impaired tissue regeneration, collagen synthesis, and immune system [4,5,6]. They may have problem in the process of wound healing which may be assisted by additional treatments, either medical or surgical treatments. Anti-inflammatory and antioxidant responses are considerably compromised in diabetes [7, 8]

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