Abstract
Ankaferd Blood Stopper® (ABS) offered as a hemostatic agent is a standardized herbal extract obtained from five different plants. The effects of ABS on colonic anastomosis are unknown. This study was designed to assess potential effects on the anastomosis of left colon in an experimental animal model. Thirtytwo male Wistar albino rats were randomized into two groups and subjected to colon anastomosis. The study group subjected to colon anastomosis with topical application of ABS to control of mucosal bleeding at the cut ends of the colon, and the control group subjected to colon anastomosis only. The rats were killed 3 and 7 days postoperatively. Four types of assessment were performed: bursting pressure, bursting wall tension, histopathology, and biochemical analysis. Compared to the control group, ABS used rats displayed a higher bursting pressure (p < 0.05) and anastomotic hydroxyproline content (p < 0.05). The use of ABS leads to a significant decrease in malondealdehyde levels (p < 0.05) and increase in paraoxonase activity (p < 0.05) at both time points. Histopathological analysis revealed that the use of ABS improves anastomotic healing in terms of reepithelialization, increased neovascularization, diminished ischemic necrosis, and inflammatory infiltration to muscle layer. Topical application of ABS to control of mucosal bleeding at the cut ends of the colon significantly improve the anastomotic wound healing by means of increasing mechanical strength and amount of tissue HPL level.
Highlights
Experimental Research Center of Selcuk toneal reflection, in all rats
Bleeding mucosa at the cut ends of the colon is a great sign of adequate perfusion, the anastomotic line must be hemostatic
Histologic changes of anastomotic wound healing, granulation tissue development, and local inflammatory response were determined according to Houdart et al.[18] and Hutschenreiter et al.[19] parameters as modified by Garcia et al.[20] (Table 1)
Summary
After anesthesia with intramuscular injecischemic necrosis, and inflammatory infiltration of ketamine hydrochloride 50 mg/kg tion to muscle layer. Topical application of ABS (Ketalar; Parke Davis, Eczacibasi, Istanbul, to control of mucosal bleeding at the cut ends of Turkey) and xylazine 5 mg/kg AG, Leverkusen, Germany), all of animals were restrained in a supine position, shaved, and 3 ical strength and amount of tissue HPL level. Animals cm midline incision was made under sterile conditions. The left colon was transected at the. This study was established at the colorectal junction, 2 cm proximal to the peri-
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