Topical Anti-inflammatory effect of aqueous extract ointment of Ageratum conyzoïdes L. in wistar rat

Abstract

Leaves of Ageratum Conyzoides commomly known as medicinal and is reported as antiinflammatory in traditional literature. So, the present research work has been entrepis with the ointment of aqueous extract of this plant for phytochemical screening and to evaluate the anti-inflammatory effect. The carrageenan induced Hind paw edema method has been used by plethysmometer. Indometacin used as reference drug and control group receive by local application valiselin. In the Carrageenan test, the ointment inhibited the first phase (200 mg/kg) and the second phase (100 mg/kg and 200 mg/kg). Also, the result of cotton pellet test shown that ointment of aqueous extract of Ageratum Conyzoides present a significantly (P<0.05) potent anti–inflammatory activity by inhibitory effect on mean increase on granuloma. Phytochemical screenings of leaves extract revealed the presence of: tannins, flavonoids, steroids, saponins and acids amines. In acute oral toxicity study, it was observed no mortality at dosesup to 5000mg/kg. The antiedematogenic effect, reduction on the exudate volume and leukocyte mobilization was observed at the doses of 100 and 200 mg/kg. The results indicated that A. Conyzoides possessed the anti-inflammatory activity; it’s confirmed this using by population.