Abstract
Aim:Tissue engineering is a multidisciplinary science that aims to produce replacement organs and biological substitutes. One of the techniques involves decellularizing a biological organ without altering its structure. One challenge is how to demonstrate which method would be better for this process.Methodology:Fifty premolar teeth were divided into five groups: G1 (control): solution of 10% formaldehyde; G2: phosphate buffer saline (PBS), 28 g of tetrasodium ethylenediaminetetraacetic (EDTA), sodium hypochlorite 2.5% (SH); G3: PBS, EDTA and 40v hydrogen peroxide (HP); G4: PBS, EDTA, SH, enzymatic detergent (ED); and G5: PBS, EDTA, HP, ED. Each group was analyzed by scanning electron microscopy (SEM), x-ray, measured weights and color and received statistical analysis.Conclusion:This study demonstrated that G5 was the most appropriate method to obtain a natural scaffold.
Highlights
This study demonstrated that G5 was the most appropriate method to obtain a natural scaffold
When the groups are compared with against G1 in terms of decellularization, the pulp was reduced in all other groups, the ones treated with hydrogen peroxide (G3 and G5)
This research demonstrated that the most appropriate method to demineralize and decellularize teeth, enabling them as natural scaffolds, was G5 (PBS, EDTA, hydrogen peroxide and Ryozime® enzymatic detergent), this presented some pulp residues in 30% of samples. Microscopic analysis demonstrated this group as the one with the least presence of biological particles, and with lower structural damage
Summary
Tissue engineering is a multidisciplinary science that aims to produce replacement organs and biological substitutes. The objective of this work is to promote a natural scaffold by using decellularizing techniques for application in tooth tissue engineering. The aim of this work is to compare and indicate which one is the most appropriate method for decellularization and structure maintenance, applied to a 50 teeth sample
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