Abstract
The Tomato yellow vein streak virus (ToYVSV) is a putative species of begomovirus, which was prevalent on tomato crops in São Paulo State, Brazil, until 2005. The objectives of this study were to evaluate the interaction between ToYVSV and its vector Bemisia tabaci biotype B and to identify alternative hosts for the virus. The minimum acquisition and inoculation access periods of ToYVSV by B. tabaci were 30 min and 10 min, respectively. Seventy five percent of tomato-test plants were infected when the acquisition and inoculation access periods were 24 h. The latent period of the virus in the insect was 16 h. The ToYVSV was retained by B. tabaci until 20 days after acquisition. First generation of adult whiteflies obtained from viruliferous females were virus free as shown by PCR analysis and did not transmit the virus to tomato plants. Out of 34 species of test-plants inoculated with ToYVSV only Capsicum annuum, Chenopodium amaranticolor, C. quinoa, Datura stramonium, Gomphrena globosa, Nicotiana clevelandii and N. tabacum cv. TNN were susceptible to infection. B. tabaci biotype B was able to acquire the virus from all these susceptible species, transmitting it to tomato plants.
Highlights
Recent studies on molecular characterization of begomoviruses infecting tomato plants (Solanum lycopersicon) indicated the existence of a complex diversity of species in Brazil (Ambrozevícius et al, 2002; Ribeiro et al, 2003), resulting from the introduction and dispersal throughout the country of the effective vector Bemisia tabaci biotype B since the 1990’s (Lourenção & Nagai, 1994; França et al, 1996)
This study aimed to investigate the acquisition, retention and transmission of a Tomato yellow vein streak virus (ToYVSV) isolate by B. tabaci biotype B, and to identify potential alternative virus hosts
Virus-free B. tabaci biotype B adults were reared on collard greens (Brassica oleraceae), soybean (Glycine max), and tomato plants, maintained in a greenhouse equipped with insect-proof screen
Summary
Recent studies on molecular characterization of begomoviruses infecting tomato plants (Solanum lycopersicon) indicated the existence of a complex diversity of species in Brazil (Ambrozevícius et al, 2002; Ribeiro et al, 2003), resulting from the introduction and dispersal throughout the country of the effective vector Bemisia tabaci biotype B since the 1990’s (Lourenção & Nagai, 1994; França et al, 1996). In tomato plants cultivated under plastic greenhouse conditions the incidence of ToYVSV ranged from 4.8% to 69.3%, depending on the planting season (Vecchia et al, 2007) This virus has been previously reported to infect green pepper (Capsicum annuum) (Nozaki, 2006) and potato (Solanum tuberosum) (Ribeiro et al, 2006; Souza-Dias et al, 1996)
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