Abstract
The pattern of protein induction in tomato plants has been investigated after the applications of pathogenic and non-pathogenic fungal species. Moreover, particular roles of the most active protein against biological applications were also determined using chromatographic techniques. Alternaria alternata and Penicillium oxalicum were applied as a pathogenic and non-pathogenic fungal species, respectively. Protein profile analysis revealed that a five protein species (i.e., protein 1, 6, 10, 12, and 13) possessed completely coupled interaction with non-pathogenic inducer application (P. oxalicum). However, three protein species (i.e., 10, 12, and 14) recorded a strong positive interaction with both fungal species. Protein 14 exhibited the maximum interaction with fungal applications, and its role in plant metabolism was studied after its identification as protein Q9M1W6. It was determined that protein Q1M1W6 was involved in guaiacyl lignin biosynthesis, and its inhibition increased the coumarin contents in tomato plants. Moreover, it was also observed that the protein Q9M1W6 takes significant part in the biosynthesis of jasmonic acid and Indole acetic acid contents, which are defense and growth factors of tomato plants. The study will help investigators to design fundamental rules of plant proteins affecting cell physiology under the influence of external fungal applications.
Highlights
Plant cell responses under the influence of pathogenic and non-pathogenic fungal species have always been an important aspect in the field of cell biology
Protein profiles of tomato cultivars were labeled by streaking images horizontally and confirmed that there were only 6 novel, defense related proteins; but 3 and 7 are presented in Figures 1A,B showing protein profiles of PC and IC treatments of Red Tara cultivar
Protein 9 was present in PC of Red Tara but it was replaced with protein 10, when same plant was inoculated with inducer species
Summary
Plant cell responses under the influence of pathogenic and non-pathogenic fungal species have always been an important aspect in the field of cell biology. It has been studied to manage plant diseases caused by viruses, bacteria and fungal pathogens (Santamarina et al, 2002; Sabuquillo et al, 2005; Islam et al, 2007; Perlin, 2009). All these studies have detected changes in biochemical profiles and protein profiles of plant cells after the application of fungal species. It is important to understand alterations in PR protein profiles after application of a pathogenic and a non-pathogenic fungal species to map out the type of changes in plant defense responses under the attack of the fungi (Turner et al, 2002; Chen et al, 2009; Kiba et al, 2012)
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