Toll-like receptor (TLR) expression on CD4 + and CD8 + T-cells in patients chronically infected with hepatitis C virus

Abstract

Toll-like receptor (TLR) expression on T-cells and the signalling pathways that lead to the production of cytokines may limit antigen-specific T-cell responses. Here, expression of TLR and retinoic acid inducible gene I (RIG-I) on T-cells were evaluated in patients chronically infected with hepatitis C virus (HCV), before and during pegylated interferon-α and ribavirin therapy. Expression of TLR2,3,4,7,9 and retinoic acid inducible gene (RIG)-I on different CD4 + and CD8 + T-cell sub-populations (naïve: CD45RA +CD57 −; central memory: T CM CD45RA −CD57 −; effector memory: T EM CD45RA −CD57 + and terminally differentiated effector memory: T EMRA CD45RA +CD57 +) were measured by flow cytometry. TLR7, TLR9 and RIG-I expression on CD4 + T-cells and RIG-I expression on CD8 + T-cells was higher in patients than healthy controls. Therapy increased expression of TLR2, TLR4 and TLR9 and this was observed for all T-cell sub-populations. Evaluation of TLR expression at baseline did not identify patients able to achieve sustained virological response following therapy.