Abstract
Abstract The recent epidemiologic studies suggest that viral infection can be one of causative factors to induce an exocrinopathy. However, the event leading to the initiation of the disease in relation with viral infection is still unknown. In this experiment, we examined whether TLR3 is expressed in human submandibular gland (HSG) cell lines and involve in chemokine release using real-time PCR, ELISA and chemotaxis experiments. We found TLR3 mRNA was expressed in this cell line. TLR3 stimulation with poly (I: C) increased mRNA expression levels of IP-10, I-TAC, and RANTES in a concentration-dependent manner. Application of neutralization antibody of TLR3 inhibited the increase of mRNA levels, suggesting it’s specifically mediated by TLR3. We confirmed the increase of chemokine concentrations in extracellular medium of HSG treated by poly (I: C). In the chemotaxis experiment, the under layer of culture media including released chemokines from poly (I: C) treated HSG cells induced T-lymphocyte migration from the upper layer of boyden chamber. Our results suggest that TLR3 functionally expressed in salivary glands and plays a role in the initiation of inflammation.
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