Toll‐like receptor 10 Expression in Chicken, Cattle, Pig, Dog, and Rat Lungs

Abstract

Toll‐like receptors (TLRs) are conserved immune receptors that play critical roles in innate immunity and are known as “gate keepers” of immune system. TLR10 is identified as type 1 plasma membrane protein but the identity of its ligand remains unclear. Till date, no data available on tissue and cell specific expression of TLR10 in normal and inflamed lungs of domestic animal species, and rat, which is commonly used as a model to study human diseases. First, we used homologous sequence alignment of published sequences of TLR10 from cattle, pig, dog, chicken and rat and the peptide alignment with antibody sequence to determine that a commercially available TLR10 antibody may be suitable for detection of TLR10. Western blotting of total lung protein extracts from cattle, dog, pig and chicken showed a band in 85‐ 100kDa region indicating the TLR10 protein. The immunohistochemistry, immunoelectron microscopy and confocal microscopy data show TLR10 expression in vascular endothelium and smooth muscle actin of control and inflamed animals. Further, we detected that basal expression of TLR10 in bovine neutrophil is altered upon treatment with E. coli lipopolysaccharide. These data show TLR10 expression in the lungs of these mammalian species and that activation of bovine neutrophils alters the expression of TLR10